【摘要】 目的:建立以反相高效液相色谱法测定消炎利尿合剂中槲皮素含量的方法。方法:色谱柱为Syltech-500C18(250mm×4.6mm,5μm),流动相为甲醇-0.2%磷酸(50∶50),流速为1.0mL.min-1,检测波长为360nm,柱温为25℃。结果:槲皮素检测浓度在19.2～96.0μg.mL-1范围内与峰面积积分值呈良好线性关系(r=0.9994);平均加样回收率为99.40%,RSD=2.31%(n=6)。结论:本方法专属性强、灵敏度高、干扰小、结果可靠,可用于消炎利尿合剂的质量控制。更多还原

【Abstract】 OBJECTIVE:To establish RP-HPLC for the determination of the content of Quercetin in the Xiaoyanliniao mixture.METHODS:Samples were Separated on Syltech-500 C18 column(250mm×4.6mm,5μm).The mobile phase consisted of methanol-0.2% phosphoric acid(50∶50)at a flow rate of 1.0mL·min-1.The detection wavelength was 360nm,and the column temperature was 25℃.RESULTS:The linear range of Quercetin was from 19.2 to 96.0μg·mL-1(r=0.999 4).The average recovery was 99.40%(RSD=2.31%,n=6).CONCLUSION:The method was specific,sensitive and reliable in results yet with little interference,and it can be used as quality control for Xiaoyanliniao mixture.更多还原