【摘要】 β-防御素主要是由哺乳动物黏膜上皮细胞产生,分布在机体宿主-环境的界面位置。在本研究中,利用已克隆的驯鹿β-防御素reBD-1cDNA作为探针,经地高辛标记后再应用原位杂交技术检测reBD-1mRNA在驯鹿组织内的表达部位。结果显示reBD-1mRNA表达在舌背表面的复层扁平上皮内,实质性器官肾也表达在肾小管及肾小囊上皮内,而淋巴器官脾脏内reBD-1mRNA表达在淋巴细胞和红髓的巨噬细胞内。表明驯鹿β-防御素不仅表达在上皮细胞内,而且在非上皮细胞内也有表达,提示reBD-1在黏膜和系统防御中均起着重要的作用。更多还原

【Abstract】 Mammalian β-defensins are produced primarily by epithelial cells lining the respiratory,digestive,and urogenital tracts and distribute the interface of organism and environment.In this study we examined the expressive tissues of reBD-1 mRNA in reindeers by RNA In Situ Hybridization using the digoxigenin-labeled reBD-1 nucleic acid probe.The reBD-1 mRNA was detected in the stratified squamous epithelium of dorsal tongue,the renal capsule and tubule epithelium of kidney and in the lymphocytes and microphages of spleen,suggesting that the reindeer β-defensin was expressed not only in epithelium but also in non-epithelial cells.The results indicate that reBD-1 may play an important role both in mucous and systemic defense.更多还原