【摘要】 目的探讨用非放射性标记的寡聚核苷酸探针(GTG)5进行近交系小鼠的DNA指纹分析。方法用非放射性标记的寡聚核苷酸探针(GTG)5制作BALB/c、C57BL/6J、DBA/2、C3H近交系小鼠的DNA指纹图,对这四个品系的小鼠进行遗传检测,分析各品系内和品系间的遗传变异性。结果(GTG)5探针可产生具有良好多态性的DNA指纹图,平均图带数为8~12条。各品系内的DNA指纹图平均相似系数(-x)在0.96~1.00的范围内,具有相同指纹图的概率(P)均在3.1×10-1以上,极显著地高于品系间的相似系数(0.22~0.39)和相同指纹图的概率(P<1.07×10-4)。结论(GTG)5可用于制作近交系小鼠的DNA指纹图以对其进行遗传检测。更多还原

【Abstract】 Objective To investigate the DNA fingerprinting of inbred strain mice generated by non-radioactive labeled oligonucleotide probes（GTG）₅.Methods DNA fingerprints of BALB/c,C57BL/6J,DBA/2,C3H inbred strain mice were generated by non-radioactive labeled oligonucleotide probes（GTG）₅ to make a genetic monitoring for these four breeds and to determine the intrabreed and interbreed variations of mouse DNA fingerprints.Results There were 8～12 distinguishable bands on every DNA fingerprinting map and each map had polymorphism.The similarity coefficient（） within breeds was 0.96～1.00,the probability of an identical DNA fingerprinting in them（P） was above 3.1×10^-1,significantly higher than that between breeds（=0.22～0.39,P<1.07×10^-4）.Conclusion The oligonucleotide probe（GTG）5 could be used in DNA fingerprinting for genetic monitoring in inbred strain mice.更多还原