【摘要】 目的观察补肾活血中药复方对糖尿病大鼠视路星形胶质细胞和少突胶质细胞的影响。方法以STZ制作大鼠实验性糖尿病模型。用免疫组化染色结合图像分析半定量测定视网膜、外侧膝状体、视皮质中胶质纤维酸性蛋白(glial fibrillary acidic protein,GFAP)和髓鞘碱性蛋白(myelin basic protein,MBP)的表达。结果与正常对照组相比,阴性对照组糖尿病大鼠视网膜、外侧膝状体及视皮质中GFAP和MBP阳性染色均不同程度的降低(P<0.05或P<0.01);与阴性对照组相比,中药各剂量组视路3个部位的GFAP、MBP阳性染色均增高,尤以中药高剂量组明显(P<0.05或P<0.01);在同等使用降糖灵的基础上,中药高剂量组视路GFAP、MBP的阳性染色较阳性对照Ⅰ组明显增高(P<0.05或P<0.01)。结论STZ性糖尿病大鼠在高血糖持续6个月后,视路3个部位的星形胶质细胞和少突胶质细胞出现免疫组织化学病理改变;补肾活血中药复方能够诱导糖尿病大鼠视路星形胶质细胞发生反应性胶质化,促进神经纤维髓鞘结构的恢复,这可能是补肾活血中药复方减轻糖尿病视功能损害的重要机制之一。更多还原

【Abstract】 OBJECTIVE To investigate the influence of traditional chinese medicine(TCM)for bushenhuoxue(补肾活血)on astroglias and oligodendrocyte of visual pathway in experimental diabetic rats.METHODS The model of experimental diabetic rats was induced by streptozotocin(STZ).Expression of glial fibrillary acidic protein(GFAP)and Myelin basic protein(MBP)in retina,exteral geniculate body(EGB)and visual cortex(VC)were shown by immunohistochemical staining,and were quantitated by image analysis system.RESULTS Compared with the normal control group,the positive staining of GFAP and MBP in retina,EGB and VC were lower significantly in negative control group(P<0.05 or 0.01);Compared with the negative control group,the positive staining of GFAP and MBP in retina,EGB and VC were upregulated in all of the TCM groups,especially in the high dose group(P<0.05 or 0.01);By using the same dose of jiangtangling(降糖灵),the positive stainning of GFAP and MBP in the high dose TCM group were obviously increased compared with the positive control group Ⅰ(P<0.05 or 0.01).CONCLUSIONS After continuous hyperglycemia for 6 months,the experimental diabetic rats showed immunohistochemical changes of astroglias and in visual pathway.TCM for bushenhuoxue could induce astroglias to generate reactive gliosis in visual pathway,promote recovery of myelin structures.Accordingly,it may be one of the important mechanisms of TCM for bushenhuoxue to relieve the damages of optical function caused by diabetes.更多还原