【摘要】 目的:观察分米波对2型糖尿病大鼠主动脉MMP-9表达的影响,探讨分米波治疗糖尿病大血管病变的可行性。方法:将60只SD大鼠随机分为4组,即正常对照组(A组)、糖尿病模型组(B组)、糖尿病罗格列酮治疗组(C组)和糖尿病分米波治疗组(D组)。应用高脂饲料加小剂量链脲佐菌素建立2型糖尿病大鼠模型,用逆转录—聚合酶链反应检测MMP-9mRNA的表达,并观察各组成模后4周、8周和12周MMP-9表达的变化。结果:糖尿病组大鼠主动脉MMP-9mRNA的表达呈增高趋势,分别是正常组的1.15、1.30、1.45和1.99倍;罗格列酮治疗组MMP-9mRNA表达量分别比模型组降低26.80%、27.16%和46.46%;分米波治疗组分别比模型组降低25.90%、26.08%和43.35%。结论:分米波照射同罗格列酮一样能降低2型糖尿病大鼠主动脉MMP-9mRNA的表达,提示分米波对糖尿病大血管病变有一定治疗作用。更多还原

【Abstract】 Objective:To explore the feasibility of decimeter wave therapy in diabetic macroangiopathy through observing the effect of decimeter wave on matrix metalloproteinase-9(MMP-9) expression.Method: Sixty SD rats were divided randomly into normal control group(group A),type 2 diabetes mellitus(T2DM)model group(group B),T2DM with rosiglitazone group(group C) and T2DM with decimeter wave group(group D). Type 2 diabetic rat model was established by high-fat diet and low dose of STZ. The MMP-9 mRNA expression in arterial wall was observed by RT-PCR. Meanwhile the changes of MMP-9 mRNA expression were observed after 4,8 and 12 weeks of T2DM respectively. Result:MMP-9 mRNA in DM rats was 1.15,1.30,1.45 and 1.99 times higher than that of control rats at week 0,4,8 and 12 after DM. MMP-9 mRNA dropped by 26.80%,27.16% and 46.46% in rosiglitazone group and 25.90%,26.08% and 43.35% after treatment for 4,8 and 12 weeks respectively.Conclusion: Similar to rosiglitazone,decimeter wave could decrease the expression of MMP-9 mRNA. It was suggested that decimeter wave may treat diabetic macroangiopathy.更多还原