【摘要】 利用Biolog微平板技术研究了水平潜流人工湿地基质微生物群落结构及代谢特性,结果表明,供试人工湿地基质微生物群落的碳源代谢能力,床体上层高于床体下层,前部高于后部.在实验期内,前部上层样点平均色度变化(AWCD)值增长速率较前部下层、后部上层及后部下层分别高出83.3%、15.8%和57.1%.主成分分析(PCA)结果表明,不同部位的基质微生物群落具有不同的群落结构和代谢特征,其对不同碳源的利用能力也存在显著性差异(P<0.05),前部上层基质微生物利用率较高的碳源为糖类和氨基酸,而前部下层对聚合物和羧酸的代谢程度最高.功能多样性指标分析表明,基质上层微生物群落丰度、均匀性和多样性指标均高于下层,前部下层微生物群落的均匀性指数显著低于其他取样点,存在较强的优势种;后部下层的丰度指数则明显低于其他采样部位.更多还原

【Abstract】 Utilizing Biolog microplate technique, the metabolic properties and functional diversity of the microbial communities in a horizontal sub-surface flow constructed wetland (HFCW) was studied. The metabolic activities of microbial community carbon source of bed body upper layer in HFCW were higher than the bed body lower layer; the front part was higher than the back part in the upper layer, but lower than the back part in the lower part. In the test period, the front upper layer sampling site average well color development (AWCD) value increase rate was 83.3%, 15.8%, and 57.1% higher than the front part lower layer, back part upper layer and back part lower layer, respectively. The result of principal component analysis (PCA) showed that the microbial community of different positions possessed each different community structure and metabolic property, its utilizing ability of different carbon source also existed marked variation (P<0.05), the carbon source of higher utilizing rate of front part upper layer were carbohydrates and amino acids, while that of highest utilizing rate of front part lower layer were polymer and carboxylic acids. The analysis of functional diversity index showed that the richness, evenness and diversity of substrate upper layer microbial communities were all than those of lower layer. The evenness index of front part lower layer microbial community was lower markedly than the other sampling sites; the richness index of back part lower layer was lower than the other sampling sites.更多还原