【摘要】 目的探讨维生素D受体(VDR)基因多态性与接铅工人血铅和血清钙的关系。方法整群抽取成都市郊区某炼铁厂接铅作业人员112人,抽取其静脉血进行血铅、血清钙和基因多态性测定,按基因型测定结果分组分别比较其血铅、血清钙的差异,并进行血铅和血清钙的相关性分析。结果112名男性接铅工人中,99人(88.4%)为VDR-bb型,10人(8.9%)为VDR-Bb型,3人(2.7%)为VDR-BB;基因型的分布符合遗传学上的Hardy-Wein-berg平衡(P=1.0)。VDR-Bb/BB型的血铅高于VDR-bb型,但血清钙低于VDR-bb型。血铅和血清钙的相关性分析结果显示,VDR-bb型的相关系数r=-0.286,P=0.004;VDR-Bb/BB型的相关系数r=-0.451,P=0.122。结论维生素D受体基因多态性对接铅工人血铅和血清钙有一定影响。更多还原

【Abstract】 Objective To study the association between VDR gene polymorphism and blood lead(PbB) and serum calcium levels in the occupationally lead-exposed workers.Methods 112 blood samples of occupationally lead-exposed workers were selected from a puddling factory in the suburb of Chengdu.An assay based on the polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) technique of restriction enzyme Bsm Ⅰ was used to detect at the same time.Results The genotype distribution in these occupationally exposed workers was 99 VDR-bb homozygotes(88.4%),10VDR Bb heterozygotes(8.9%),3VDR-BB homozygotes(2.7%).The distribution of genotype was coincided with Hardy-Weinberg balance(P=1.0).The levels of PbB of VDR-Bb heterozygotes and VDR-BB homozygotes were higher than VDR-bb homozygotes,but the level of serum calcium of the former was lower than the later.The correlation coefficient of PbB and secrum calcium of VDR Bb heterozygotes and VDR-BB(r=0.4510,P=0.122) homozygotes were higher than VDR-bb(r=0.286,P=0.004) homozygotes.Conclusion The polymorphism of VDR gene is associated with blood lead and serum calcium.更多还原