【摘要】 目的通过观察MAN2C1转基因小鼠对H5N1高致病性禽流感病毒的易感性,以了解此转基因小鼠的免疫应答情况和MAN2C1基因在病毒感染中的作用。方法以H5N1高致病性禽流感病毒滴鼻感染MAN2C1转基因小鼠,HE染色观察小鼠肺组织病理变化;RT-PCR和免疫组织化学方法检测小鼠肺组织H5N1病毒载量;间接ELISA方法检测小鼠血清抗体滴度变化。结果与对照组小鼠比较,MAN2C1转基因小鼠表现为更为严重的间质性肺炎,肺组织病毒载量增加,外周血中性粒细胞数目降低,淋巴细胞数量增加,血清IgG抗体滴度降低。结论MAN2C1基因抑制了小鼠的体液免疫。更多还原

【Abstract】 Objective To study the immunologic function of MAN2C1 gene in the transgenic mice.Methods Infection of transgenic mice with H5N1 were performed by nasal dripping.The pathological changes of lung tissues were observed by HE staining and immuno-histochemistry.H5N1 viral load in lung of mice were detected with RT-PCR.Serum antibody against H5N1 was measured with indirect ELISA.Results The pathological changes were more serious in the transgenic lung than that of control mice.The expression levels of H5N1 virus antigen and virus load in the lung tissues of transgenic mice were obviously higher than that in control mice. A decreased level of serum antibody against H5N1 was also observed in the transgenic mice compared with control mice.Conclusion Our results suggest that MAN2C1 gene down-regulates the humoral immunity against virus infection.更多还原