【摘要】 目的分析不同浓度氧乐果对运动神经元agrin基因mRNA转录水平的影响,探讨有机磷中毒突触前损害的发病机制。方法体外培养大鼠脊髓运动神经元,氧乐果染毒,检测不同浓度氧乐果对运动神经元agrin基因mRNA转录水平的影响,检测有机磷中毒是否对运动神经元的agrin基因表达产生影响。结果(1)正常组大鼠脊髓运动神经元agrin基因mRNA水平明显高于氧乐果染毒组大鼠脊髓运动神经元agrin基因mRNA水平;(2)不同浓度氧乐果染毒组(0.1mmol/L,0.01mmol/L)大鼠脊髓运动神经元agrin基因mRNA水平,各组间存在显著差异,损害程度与有机磷杀虫剂剂量存在正相关,即浓度大,agrin基因转录水平低;(3)0.1mmol/L浓度氧乐果染毒组大鼠脊髓运动神经元存活时间小于12h,0.01mmol/L浓度氧乐果染毒组大鼠脊髓运动神经元可较长时间存活。结论有机磷杀虫剂影响脊髓运动神经元agrin基因表达,且损害程度与有机磷杀虫剂剂量存在正相关,即浓度大,agrin基因转录水平低,神经肌肉接头损害严重,存在突触前损害。更多还原

【Abstract】 Objective This research aims to identify the relationship between organophosphate posioning and forming and maintenance of neuromuscular junction.Thus discuss further the pathogenesis of intermediate syn- drome of pre-synaptic neurons.By analyzing the effects of omthoatc on the mRNA detected transcriptional level of agrin gene,the probability of organophosphate poisoning affecting agrin gene of the dynamoneure will he concluded here.Methods Organophosphorus pesticide was used to interfere with the rat dynomoneure cultured in vitro.Con- sequently,the effects of omethoate on the mRNA transcriptional level of angrin gene of rat dynamoneure was andysde.The problem whether organophosphate poisoning affects the level of agrin gene of the motor neuron was determined.Results (1)The mRNA transcripitional level of agrin gene in the group of normally cultured rat dyna- moneure was higher than that of the group poisoned with omethoate.(2)There was significant deviation in different groups with different density of omethoate poisoning.The transcriptional level of agrin gene in 0.1mmol/L omethoate poisoning motor neuron was reduced compared with that of dynamoneure in 0.01mmol/L omethoate poisoning.And there was positive correlation between damage degree of dynamoneures and the dose of organophos- phorus pesticide.(3)The 0.1mmol/L omethoate poisoning motor neurons died in 12 hours after omethoate was used.While 0.01mml/L omethoate poisoning motor neurons survived a long time.Conclusion Organophosphorus pesticide has an predominant effect on the transcription of agrin gene.There is positive correlation between the lev- el of damage and the concentration of the organophosphate pesticide.更多还原