【摘要】 目的观察酪蛋白(CA)对高碘小鼠甲状腺细胞凋亡的影响。方法将昆明种小鼠随机分为4组:对照(NI)组、高碘(HI)组、高碘+CA1(HI+CA1)组、高碘+CA2(HI+CA2)组,喂养12个月,ELISA法检测血清白细胞介素1β(IL-1β),RT-PCR法检测甲状腺组织Fas、FasL mRNA表达水平,TUNEL法检测甲状腺细胞凋亡。结果与NI组比较,HI组小鼠血清IL-1β水平和甲状腺组织Fas mRNA表达均明显增高(P<0.05或<0.01),且甲状腺滤泡上皮凋亡细胞数也明显增多(P<0.01),而高碘小鼠随着CA摄入量增高,上述改变程度明显降低。结论高碘可通过提高机体IL-1β水平,诱发凋亡调控基因Fas/FasL系统表达改变,促进大鼠甲状腺细胞凋亡。而CA摄入可以部分拮抗高碘引起的上述改变,对滤泡上皮细胞具有一定的保护作用。更多还原

【Abstract】 Objective To observe the effects of casein (CA) on apoptosis of mice thyroid under excessive iodine.Methods Kunming mice were divided into 4 groups:normal control(NI) group,high iodine(HI) group, high iodine plus CA1 (HI+CA1) group and high iodine plus CA2 (HI+CA2) group.After twelve months,we examined the levels of IL-1βin the serum by ELISA,thyroid apoptosis by TUNEL,and Fas,FasL mRNA by RT- PCR.Results Compared with that in NI,the levels of IL-1βin the serum,Fas mRNA expression and cell apoptosis of thyroid increased markedly in HI(P<0.05 or<0.01),however they decreased in the high iodine plus CA group.Conclusions Iodine excess may elevate IL-1βin the serum and stimulate Fas/FasL system expression of thyroid follicles epithelial cell,leading to thyroid cell apoptosis,which can be protected by CA protein.更多还原