【摘要】 目的:建立心宁片(丹参、三七、红花、川芎等)中三七皂苷R1、人参皂苷Rg1、Rb1的含量测定方法。方法:采用HPLC梯度洗脱法,使用C18柱,流动相为乙腈-水梯度洗脱,(0～5min,20～25乙腈;5～20min,25～45乙腈);ELSD漂移管温度70℃;氮气流速2.0L/min;柱温35℃。结果:3种皂苷类成分得到很好分离,线性关系良好,平均加样回收率:三七皂苷R1,人参皂苷Rg1,人参皂苷Rb1分别为102.32,100.73和101.40。RSD分别为0.97,1.16和1.21。结论:本法结果准确,便于操作,可作为心宁片的质量控制方法之一。更多还原

【Abstract】 AIM:To establish the method of determining notoginsenoside R₁,ginsenoside Rg₁ and Rb₁ in Xinning Tablet（Radix et Rhizoma Salvae Miltiorrhizae,Radix et Rhizoma notoginseng,Flos Carthami,Rhizoma Chuanxiong,ect）.METHODS:HPLC-ELSD was used to determine notoginsenoside R₁,ginsenoside Rg₁ and Rb₁ in Xinning Tablet.The separatrion was performed on C 18 colunm with acetonitrile and water being used as a gradient program at 35 ℃.The elution program was（0-5 min,20%-25% acetonitrile;5-20 min,25%-45% acetonitrile）,drift tube temperature was at 70 ℃,gas flow rate of 2.0 L/min.RESULTS:3 saponins were separated well.Average recoveries were 102.32% for notoginsenoside R₁ 100.73% for ginsenoside Rg₁;101.40% for ginsenoside Rb₁,respectively.CONCLUSION:The method is simple and rapid and with satisfactory results and is suitable for quality control of Xinning Tablet.更多还原