【摘要】 目的:建立RP-HPLC法同时测定复方沙棘籽油栓(沙棘籽油,蛇床子,苦参等)中蛇床子素和欧前胡素的含量的方法。方法:色谱柱为Phenomnex(luna)C18(4.6 mm×250 mm,5μm),柱温:25°C;流动相为甲醇-水(65∶35),流速:1.0mL/min;检测波长:310 nm。结果:蛇床子素和欧前胡素分别在0.051~0.816μg(r=0.999 6)和0.026~0.416μg(r=0.999 8)范围内线性关系良好,平均回收率分别为98.4%和97.6%,RSD分别为1.4%(n=6)和2.0%(n=6)。结论:本法简便、结果准确、重现性好,可用于复方沙棘籽油栓中蛇床子素和欧前胡素的含量测定。更多还原

【Abstract】 AIM:To develop a HPLC method of determining osthole and imperatorin in Compound Shajiziyou Suppository（oil of Semen Hippophae,Fructus Cnidii,Radix Sophorae flavescentis,etc.）. METHODS: Phenomnex（luna） C₁₈ column was used at（25 °C）.The mobile phase consisted of methanol-water（65（∶）35）.The flow rate was 1.0 mL/min.The detection wavelength was set at 310 nm. RESULTS: The linear ranges of osthole and imperatorin were 0.051-0.816 μg（r=0.999 6） and 0.026-0.416 μg（r=0.999 8）,respectively,The average recoveries were 98,4% with RSD of 1.4% and 97.6% with RSD of 2.0% respectively. CONCLUSION: This method is simple,accurate,reproducible and can be used for the determination of osthole and imperatorin in Compound Shajiziyou Suppository.更多还原