【摘要】 目的:观察柴胡皂甙d(SSd)对新西兰黑色品系和白色品系杂交的子一代(NZBWF1)狼疮肾炎小鼠尿蛋白含量及肾组织IL-6、IL-10、TNF-α、IFN-γmRNA表达的影响。方法:将雌性NZBWF1小鼠分为3组:①SSd治疗1组(SSd剂量2mg·kg-1·d-1);②SSd治疗2组(SSd剂量4mg·kg-1·d-1);③对照组;共治疗6周。小鼠尿蛋白含量用考马斯亮蓝法检测,小鼠肾组织IL-6、IL-10、TNF-α、IFN-γmRNA表达用逆转录-聚合酶链反应(RT-PCR)法检测。结果:实验2周、4周以及6周末,与对照组相比,SSd治疗1组和SSd治疗2组尿蛋白含量明显降低(P<0.05);与SSd治疗1组相比,SSd治疗2组尿蛋白含量显著降低(P<0.05)。实验6周末,SSd治疗1组和SSd治疗2组肾组织IL-6和IL-10 mRNA表达水平明显低于对照组(P<0.05);SSd治疗2组IL-6和IL-10 mRNA表达水平显著低于SSd治疗1组(P<0.05)。SSd治疗1组、SSd治疗2组和对照组相比,肾组织TNF-α、IFN-γ mRNA表达水平之间的差异无统计学意义(P>0.05)。结论:SSd可以降低NZBWF1狼疮肾炎小鼠尿蛋白含量并抑制肾组织IL-6、IL-10 mRNA的表达,而对肾组织TNF-α、IFN-γ mRNA的表达无影响。更多还原

【Abstract】 AIM: To observe the effects of saikosaponin-d(SSd) extracted from Bupleurum falcatum L. on urine protein content and expression of IL-6,IL-10,TNF-α,IFN-γ mRNA in renal tissue of NZBWF1 mice. METHODS: 20 famale NZBWF1 mice, eighteen weeks old, were randomized into three groups:(1) SSd treatment 1st group [intraperitoneal injection(ip) SSd 2 mg·kg-1·d-1];(2) SSd treatment 2nd group (ip SSd 4 mg·kg-1·d-1); (3)Blank control group.The experiment lasted for 6 weeks. Urine protein content in NZBWF1 mice was detected using the method of Coomassie brilliant blue. Expressions of IL-6,IL-10,TNF-α,IFN-γ mRNA in renal tissue of NZBWF1 mice were detected using reverse transcription-polymerase chain reaction (RT-PCR). RESULTS: At the end of the 2,4,6 weeks of experiment, compared with the control group, urine protein content was decreased significantly in the first and second group of SSd treatment(P<0.05). Compared with the first group of SSd treatment, urine protein content was reduced remarkably in the second group of SSd treatment(P<0.05). At the end of the 6 weeks of experiment, compared with the control group, expression of IL-6,IL-10 mRNA in renal tissue was diminished significantly in the first and second group of SSd treatment(P<0.05). Compared with the first group of SSd treatment, the expression of IL-6,IL-10 mRNA was decreased remarkably in the second group of SSd treatment(P<0.05). The differences of expression of TNF-α,IFN-γ mRNA among three groups were not statistically significant (P>0.05). CONCLUSION: Urine protein content and expressions of IL-6,IL-10 mRNA in renal tissue of NZBWF1 mice can be reduced by SSd treatment,while expressions of TNF-α,IFN-γ mRNA in renal tissue are not affected.更多还原