【摘要】 目的:探讨环境压力对中国树鼩生殖生理的影响。方法:中国树鼩30只,随机分为3组,1组为对照组10只,2组8只,3组12只。实验组采用缩小饲育环境、增大同种性别动物密度的方法,用放免仪测定性激素水平,用光镜观察睾丸的组织结构。结果:经分笼饲养21d测定,2、3组动物血清睾酮(T)比1组分别低49.4%和80.3%(P<0.01);雌三醇(E2)水平各组间差异无显著;光镜观察发现,2组动物睾丸组织曲细精管在高倍镜下可见精原细胞与支持细胞网架;3组仅见少数几个精原细胞。结论:环境压力能抑制中国树鼩睾酮形成,损伤睾丸组织结构。更多还原

【Abstract】 AIM:To study the effect of environment stress on reproductive physiology in Chinese tree shrew.METHODS:30 animals were divided at random into three groups.Group 1:10 of 30 animals served as control;group 2 and 3 the 8 and 12 of 30 animals served as experiment and housed in small cage.The levels of the testosterone(T)and estradiol(E2)were determined by the method of radioimmunoassay and section preparation of testis was observed by light microscope after living in the reduced scope for 21days.RESULTS:The result showed that the levels of the testosterone in group 2 and 3 were decreased by 49.4% and 80.3%,respectively,compared to that in group1.The convoluted tubules of testis in group 2 and 3 were damaged and only a few spermatogonia was doserved.CONCLUSION:Social environment stress has significantly inhibitory effect on testosterone formation and destroy the testis structure of Chinese tree shrew.更多还原