【摘要】 为研究温度骤变在水生动物病害发生中的可能作用,本研究以中华绒螯蟹为材料,将环境温度从20℃突然升高或下降,然后在不同时间取样分析,从免疫学的角度探讨了不同的温度改变方式对中华绒螯蟹抗氧化能力的影响.研究结果表明,(1)在2h内将饲养水温从20℃升至32℃,然后在30min内降至20℃,中华绒螯蟹的血细胞密度(DHC)、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活力先升高后下降,6h时出现峰值,3d后趋于稳定,丙二醛(MDA)的含量变化则呈相反趋势;(2)在24h内将饲养水温从20℃降至8℃,其DHC随时间的延长而逐渐降低,SOD和CAT先增加后下降,在d1出现峰值;而MDA的含量先下降后升高,在d3出现峰值,但总的来说,MDA的含量均高于0h处理组;(3)在24h内水温从20℃升至32℃,其SOD和CAT在温度变化6h后呈升高趋势,而后逐渐下降,DHC的变化无一定规律,但均明显低于0h处理组,MDA的含量随时间延长而增加.以上结果表明,温度胁迫使中华绒螯蟹机体抗氧化能力下降,从而导致机体的氧化代谢失衡和免疫防御能力下降.更多还原

【Abstract】 Temperature stress could be one of the main factors for disease outbreak. This study focused on the immunological response of adult female Eriocheir sinensis to different temperature changes in order to understand how the stress modulated the immunology and defense system of E. sinensis females. Some blood immunochemical variations were used as indicators of stress measured during the 9-day experiment. The results indicated: (1)After ambient temperature reached 32 ℃ from 20 ℃ in 2 h and then dropped to 20 ℃ in 30 min, the density of haemocyte cells (DHC), superoxide dismutase (SOD) activity and catalase (CAT) activity in crabs increased, then decreased, peaked at 6 h, and leveled off after day 3, while malodiadehyde (MDA) level was opposite compared with the DHC, SOD and CAT; (2) After ambient temperature decreased to 8 ℃ from 20 ℃ in 24 h, the DHC decreased gradually, SOD and CAT activity increased, and then decreased and peaked at day 1, while MDA level decreased, and then increased and peaked at day 3, and totally the MDA level after stress was higher than that in 0 h treatment; (3) 6 h after ambient temperature reached 32 ℃ from 20 ℃ in 24 h, SOD and CAT activity increased, and then decreased gradually. The DHC level after stress was lower than that in 0 h group, but the change in DHC was instable. Also the MDA content increased with time elapsed. Therefore, the results indicated that temperature changes depressed the ability of anti-oxidation, disrupted the balance of metabolism of reactive oxygen radical and reduced the resistance to disease.更多还原