【摘要】 目的通过接种转染携带小鼠内皮抑素基因的腺病毒到人血管内皮细胞(ECV304),观察内皮抑素基因表达对人血管内皮细胞增殖的影响。方法分对照Ⅰ组(磷酸盐缓冲液)、对照Ⅱ组(携带绿色荧光蛋白基因的重组腺病毒)和实验组(携带小鼠内皮抑素基因的腺病毒),用二苯基四氮唑溴盐法(MTT法)测定各孔的光吸收值,计算细胞增殖抑制率。结果各组的细胞增殖抑制率分别为7%(对照Ⅰ组)、6%(对照Ⅱ组)和78%(实验组)。结论证实腺病毒携带的内皮抑素基因可以抑制人血管内皮细胞系的增殖,为进一步研究内皮抑素基因治疗提供实验基础。更多还原

【Abstract】 Objectives To observe the influence of endostatin gene expression on t he proliferation of human endothelial cell(ECV304)by infecting adenovirus wi th mouse endostatin gene.Methods All cells were divided into three groups,which are first control group with PBS,second control group with recombinant adenovirus carrying green fluorescent protein(GFP)and experime ntal group with adenovirus carrying mouse endoststin gene.cell proliferative inhibition ratio were calculated according to photoabsorption value in every well by microculture tetrazolium dye(MTT)assay.Results T he cell proliferative inhibition ratio of 3 groups were 7%(first C ontrol group),6%(second Control group)and 78%(experimental group)respec tively.Conclusions The endostatin gene carried by adenovir us can inhibit the proliferation of ECV304,which provided experimental basis f or further gene therapy of endostatin gene.更多还原