【摘要】 为研究大黄素(emodin)对人髓系白血病细胞株HL-60细胞增殖、凋亡的影响及Akt信号通路在其中的作用,应用MTT法检测大黄素对HL-60细胞增殖的影响;细胞周期分析、线粒体细胞凋亡流式检测法分析细胞周期变化及细胞凋亡;Western blotting检测Akt信号通路蛋白表达水平的变化。结果显示,大黄素能有效抑制HL-60细胞的增殖,作用48 h的IC50约为20μmol.L-1,并能诱导其凋亡,随药物作用浓度的增加,凋亡率也逐渐上升;大黄素作用后,HL-60细胞G0/G1期细胞增多,而S期及G2期的细胞减少;Western blotting检测结果显示,大黄素下调HL-60细胞Akt、p-Akt、IκB-α、p-IκB-α、p65、p-p65、mTOR及p-mTOR蛋白的表达。因此,大黄素能有效抑制HL-60细胞增殖,将细胞阻滞于G0/G1期,诱导其凋亡;Akt信号通路可能参与了大黄素抑制HL-60细胞增殖、诱导凋亡的过程。更多还原

【Abstract】 This study is to investigate the effect of emodin on inducing human myeloid leukemia cell line HL-60 apoptosis and the role of Akt signal pathway in the apoptosis.HL-60 cells were exposed to various dosages of emodin.MTT assay was used to detect HL-60 cell proliferation.Distribution of HL-60 cells in cell cycle was analyzed by flow cytometry and cell apoptosis was observed by MitoCapture apoptosis detection.The protein expressions of Akt signal pathway were detected by Western blotting.The result showed that emodin remarkably inhibited the cell proliferation.The IC₅₀ value for 48 h treatment was about 20 μmol·L^-1.Apoptosis in HL-60 cells could be efficiently induced by emodin in a dose dependent manner and cells were arrested at G₀/G₁.The expressions of Akt,p-Akt,IκB-α,p-IκBα,p65,p-p65,mTOR and p-mTOR in Akt signal pathway were downregulated after emodin treatment.It can be concluded that emodin could efficiently induce growth inhibition and apoptosis in HL-60 cells.Akt signal pathway may be involved in this process.更多还原