【摘要】 目的:建立紫外-三波长反相高效液相色谱法测定止血海绵中3种有效成分地塞米松磷酸钠、替硝唑、克林霉素的含量。方法:色谱柱为 Discovery C₁₈柱（150mm×4.6 mm,5μm）;流动相为乙腈-0.05 mol·L^-1磷酸二氢钾溶液-三乙胺（37:63:0.3）,用磷酸调 pH 至5.3±0.05;流速1.0 mL·min^-1;检测波长地塞米松磷酸钠为240nm,替硝唑为316 nm,克林霉素为210 nm。结果:被测3种成分在其各自最佳吸收波长处测定,可提高测定的灵敏度和准确度。地塞米松磷酸钠、替硝唑和克林霉素的平均回收率（n=15）分别为99.5%,98.9%,100.4%。结论:本法简便,结果准确,重复性好,专属性高,可作为该制剂的质控方法。更多还原

【Abstract】 Objective:To establish an RP-HPLC method for determine of 3 components in hemostatic spongea un- der 3 different UV wavelengths.Methods:The column was Discovery C₁₈（150 mm×4.6 mm,5 μm）;The mobile phase was acetonitrile-0.05 mol·L^-1potassium dihydrogen phosphate-triethylamine（37:63:0.3）,adjusted to pH 5.3 ±0.05 with phosphoric acid;The flow rate was 1.0 mL·min^-1.The detecting wavelengths was 240 nm for dexamethasone sodium phosphate,316 nm for tinidazole and 210 nm for clidamycin.Results:The result showed that when determination was finished by RP-HPLC under the respective optimum wavelength,the 3 components in hemostatic spongea were precisely quantified.The average recoveries（n=15）of dexamethasone sodium phosphate, tinidazole,and clidamycin were 99.5%,98.9% and 100.4%,respectively.Conclusion:The proposed method is a simple,accurate,reproducible and credible quality control methed for the hemostatic spongea.更多还原