【摘要】 目的:建立同时测定四季青叶中原儿茶酸、咖啡酸、槲皮素和山奈酚4种成分含量的高效液相色谱-电化学（HPLC-ECD）法。方法:采用 Zorbax SB-C₁₈（150 mm×4.6 mm,5.0 μm）色谱柱;以（A）甲醇、（B）水-0.1%醋酸为流动相,梯度洗脱:0min 时 A-B（20:80）,9 min 时 A-B（24:76）,30 min 时 A-B（50:50）,40 min 时 A-B（65:35）;流速为0.8 mL·min^-1,柱温为30℃,电化学检测器的工作电位为0.7 V,采用标准曲线法对4种化合物进行定量。结果:在选定的色谱条件下原儿茶酸、咖啡酸、槲皮素和山奈酚的检出限分别为3,6,2,5 ng,定量限分别为9.1,20.0,7.2,16.0 ng。4种化合物的平均加样回收率均在95.7%～102.6%之间,RSD 在1.6%～2.9%之间。结论:方法简便、准确,可用于同时检测四季青叶中4种化合物的含量。更多还原

【Abstract】 Objective:To establish an HPLC-ECD method for simultaneous determination of the contents of proto- catechuic acid,caffeic acid,quercetin and kaempferol in leaves of Ilex purpurea Hassk..Method:The separation was performed on Zorbax SB-C₁₈column（150 mm×4.6 mm,5.0 μm）by gradient elution with（A）methanol- （B）water containing 0.1% acetic acid[0 min,A-B（20:80）;9 min,A-B（24:76）;30 min,A-B（50:50）; 40 min,A-B（65:35）]as the mobile phase at a flow rate of 0.8 mL min^-1.The detection was done at 0.7 V and 30℃.Calibration curves were applied to the determination of four compounds.Results:The limits of detection were 3 ng for protocatechuic acid,6 ng for caffeic acid,2 ng for quercetin and 5 ng for kaempferol and the limits of quan- tification were 9.1 ng for protocatechuic acid,20.0 ng for caffeic acid,7.2 ng for quercetin and 16.0 ng for kaempferol.The average recoveries of these compounds were between 95.7%-102.6% with RSDs of 1.6%- 2.9%.Conclusion:The method is simple,rapid,reproducible and accurate.It can be used for the routine analysis of the herb of Ilex purpurea Hassk..更多还原