【摘要】 目的:建立 RP-HPLC 法测定胆汁中酮洛芬Ⅱ相代谢物酮洛芬葡萄糖醛酸（S-KPG、R-KPG）。方法:采用 ODS 柱（5μm,4.6 mm×250 mm）;流动相为乙腈-水（35:65）,内含0.05 mol·L^-1磷酸二氢钾和0.005 mol·L^-1的四丁基溴化胺,pH5.6;流速为1 mL·min^-1;检测波长232 nm;柱温30℃;进样是20μL。内标为10 mg·L^-1的萘普生甲醇溶液。结果:在2～1000μmol·L^-1浓度范围内,胆汁中 S-KPG、R-KPG 回归方程分别为 Y=0.0039X-0.0256,r=0.9998和 Y=0.0039X-0.0229,r=0.9998;最低检测限分别为0.5 μmol·L^-1和1 μmol·L^-1。S-KPG 和 R-KPG 在室温和胆汁生理 pH 时不稳定,在 pH4时最稳定。结论:本文应用 RP-HPLC 检测胆汁 S-KPG、R-KPG,方法简便、易行、实用,可以作为肝脏葡萄糖醛酸转移酶活性和胆排泄实验研究的分析手段。更多还原

【Abstract】 Objective:To establish an RP-HPLC method to determine ketoprofen glucuronides（S-KPG and R- KPG）in bile liquid.Methods:The chromatographic separation was performed using an ODS analytical column.The mobile phase consisted of acetonitrile and 0.05 mol·L^-1phosphate buffer（pH 5.6）including 5 mmol·L^-1tetra- butylammonium bromide（35:65）.The detector wavelength was 232 nm,the flow rate was 1 mL·min^-1,the volume of injection was 20 μL,and the oven temperature was 30℃.The internal standard was naproxen dissolved by meth- anol.Results:For both kinds of KPGs,the linear range was 2～1000 μmol·L^-1.The linear equation of S-KPG was Y=0.0039X-0.0256,r=0.9998;the linear equation of R-KPG was Y=0.0039X-0.0229,r=0.9998. KPGs were easy to be hydrolyzed in the condition of room temperature and bile physiological pH.However,KPGs were stable in the condition of pH adjusted to 4.Conclusion:The method is simple and utility.And it can be used for the empirical study of the glucuronyltransferase activity and bile excretion function.更多还原