【摘要】 目的:建立一种测定苏合香微丸主要成分肉桂酸含量的新方法,提出以酚酞为内标的新思路。方法:采用ZORBAX SE-C₁₈柱（250mm×4.6mm,5μm）,甲醇-水-冰醋酸（50∶50∶0.5）为流动相,检测波长242nm,流速1.5mL·min^-1,柱温室温。结果:当肉桂酸的浓度在6.4～54.4μg·mL^-1范围时,其峰面积与浓度的线性关系良好（r=0.9999）;高、中、低3种浓度的回收率分别为99.3%,99.2%,101.0%,RSD分别为1.5%,1.8%,0.9%。结论:方法准确,可靠,可作为含苏合香类药品制订质量标准时的参考。更多还原

【Abstract】 Objective:To establish a new RP-HPLC method for determination of cinnamic acid in styrax micropills, which is efficient on cardiovascular diseases, taking phenolphthalein as internal standard. Method:The determination by RP-HPLC was carried out using ZORBAX SB-C₁₈ column （250mm×4.6mm,5μm） at room temperature,with mobile phase of methanol-water-glacial acetic acid （50: 50: 0.5） at a flow rate of 1.5mL·min^-1 and the detection wavelength of 242nm. Results: Excellent linear correlation was established within the range of 6.4-54.4μg·mL^-1, and r=0.9999; The recovery of high concentration（120% ）was 99.3%, RSD was 1.5%, middle concentration （100%） was 99.2% , RSD was 1.8%, low concentration （80%） was 101.0%, RSD was 0.9%. Conclusion :The method can be applied in quantitive determination of preparations contained styrax with accuracy and reliability.更多还原