【摘要】 目的:建立一种中药中砷的形态分析方法,分离并测定雄黄及含雄黄中成药中4种形态的砷化合物:无机 As(Ⅲ)、As(V)和有机单甲基胂酸(MMA)、二甲基胂酸(DMA)。方法:以稀盐酸溶液(pH 2)浸提雄黄及含雄黄中成药中的可溶性砷,用阴、阳离子固相萃取小柱进行分离,氢化物发生-原子荧光光谱法测定4种形态砷含量。结果:4种形态砷能实现完全分离,测定精密度、准确度良好。结论:本方法较为简便,准确、可靠,适用于分离测定雄黄及含雄黄的中成药中4种形态砷含量。更多还原

【Abstract】 Objective:To develop a method for analysis of arsenic species,i.e.arsenite As（Ⅲ）,arsenite As（Ⅴ）, monomethylarsonic acid（MMA）,and dimethylarsinic acid（DMA）in Realgar and in Chinese traditional patent medicine containing Realgar.Method:Arsenic species in samples were extracted with dilute hydrochloric acid solu- tion（pH2）and the separation were performed on a silica-based strong cation-exchange（SCX）cartridge,and a resin-based strong anion-exchange（SAX）cartridge.Then hydride generation atomic fluorescence spectrometry was used to determine the speciation of arsenic in samples.Results:Calibration curves were linear with regression coeffi- cients of more than 0.999 for all four arsenic species,and the linear ranges of As（Ⅴ）、MMA and DMA were 0-20 ng·mL^-1and of As（Ⅲ）was 0-40 ng·mL^-1.The average recoveries of all four arsenic species were ranged from 81% to 105.4% and precision（RSD）were within 8.7% for DMA,12% for MMA,4.7% for As（Ⅴ）and 3% for As（Ⅲ）.Conclution:The proposed method is simple,accurate and is suitable for As speciation analysis in Realgar and in Chinese traditional patent medicine containing Realgar.更多还原