【摘要】 目的:考察不同品种、不同采摘时间和不同种植基地桑叶中绿原酸、芦丁和异槲皮苷的含量差异。方法:采用反相高效液相色谱法,以 Zorbax SB C₁₈（250 mm×4.6 mm,5μm）为分离柱,90%乙腈（A）-0.2%磷酸溶液（B）为流动相进行梯度洗脱[0 min,A-B（12:88）;10 min,A-B（16:84）;20 min,A-B（20:80）;30 min,A-B（25:75）],流速1.0 mL·min^-1,检测波长358 nm。结果:不同种植基地的桑叶中绿原酸、芦丁和异槲皮苷的含最有一定的差异;不同品种和采摘时间的桑叶中上述三成分含量差异较大。结论:桑叶中绿原酸、芦丁、异槲皮苷的含量与种植基地、采摘季节、品种等有关,其中尤以季节和品种影响较大。更多还原

【Abstract】 Objective:To compare the content of chlorogenic acid,rutin and isoquercitrin in Folium Mori from dif- ferent collection time,varieties and plant sites.Method:The contents of chlorogenic acid,rutin and isoquercitrin were determined by RP-HPLC.Zorbax SB C₁₈column（250mm×4.6mm,5μm）was used as analysis column,the mobile phase was 90% acetonitrile（A）-0.2% phosphoric acid（B）with gradient elution[0 min,A-B（12:88）; 10 min,A-B（16:84）;20 min,A-B（20:80）;30 min,A-B（25:75）].The flow rate was 1.0 mL·min^-1,the detection wavelength was set at 358 nm.Result:The contents of three components changed at some degree in Foli- um Mori from different plant sites,while the contents varied obviously among Folium Mori from different collection time and different varieties.Conclusion:The contents of three components are influenced by collected time,plant site,and varieties,especially by collected season and varieties.更多还原