【摘要】 目的:研究用固相萃取预分离,高效液相色谱内标法测定冬虫夏草中甘露醇的含量。方法:冬虫夏草样品中的甘露醇用水超声振荡浸取,浸取液用 Waters Sep-Pak-C₁₈固相萃取小柱预分离,以海藻糖为内标物,Waters Sugar-Pak-1（6.5 mm×300 mm）钙型阳离子交换柱为固定相,0.05 g·L^-1EDTA 钙钠水溶液为流动相,流速0.5 mL·min^-1,示差折光仪为检测器测定冬虫夏草样品中的甘露醇含量。结果:最低检测限为2.0μg·mL^-1;回收率在97%～102%之间,RSD 在0.86%～1.2%之间。结论:方法简便、灵敏、准确、适用于冬虫夏草样品的甘露醇测定。更多还原

【Abstract】 Objective:Study on pre-separation with solid phase extraction,and HPLC method for the determina- tion of mannitol in Cordyceps sinensis（Berk.）Saec.Methods:The mannitol was extracted from Cordyceps sinensis sample with water,and purified by solid phase extraction with Sep-Pak-C₁₈ cartridge.With fucose as internal standard,the mannitol was separated on a Waters Sugar-Pak-1（6.5 mm×300 mm）cations ion exchange column with 0.05 g·L^-1 EDTA calcium disodium salt solution as mobile phase,and flow rate was 0.5 mL·min^-1,and detected with refractive index detector system.Results:The limit of detection of mannitol was 2.0 μg·mL^-1.The mean recovery was between 97% and 102% with RSD between 0.86% and 1.2%.Conclusion:This method is simple,convenient,sensitive,accurate and suitable for the determining of the different mannitol in Cordyceps sinen- sis.更多还原