【摘要】 目的:用胶束电动毛细管色谱法分离测定三七中人参皂苷 Rg₁、人参皂苷 Re 和三七皂苷 R₁ 3种皂苷含量。方法:采用Beckman MDQ 电泳仪,40 cm×50 μm的熔融石英毛细管,有效柱长30 cm,缓冲液为20 mmol·L^-1SDS、40 mmol·L^-1胆酸钠、60 mmol·L^-1硼砂,检测波长200 nm。结果:人参皂苷 Rg₁低、中、高剂量的平均加样回收率分别为101.5%,100.6%,100.4%,RSD 分别为2.4%,2.2%,1.0%;人参皂苷 Re 低、中、高剂量的平均加样回收率分别为101.3%,99.23%,99.66%,RSD 分别为2.0%,1.3%,1.1%;三七皂苷 R₁低、中、高剂量的平均加样回收率分别为100.6%,100.5%,100.7%,RSD 分别为1.1%,1.1%,0.86%。结论:本方法能够将三七药材中人参皂苷 Rg₁、人参皂苷 Re 和三七皂苷 R₁完全分离,具有灵敏度高、快速、易于重现且所用试剂价格低廉的特点,因此适合于中药三七的测定,并对今后三七的鉴别和质量控制具有重要意义。更多还原

【Abstract】 Objective:To separate and determine ginsengnoside Rg₁,ginsengnoside Re and notoginsengnoside R₁ from Radix et Rhizoma Notoginseng.Method:Experiments were performed on Beckman P/ACE MDQ capillary e- lectrophoresis system,the fused-sillica capillary tube was used with 20 mmol·L^-1 SDS,40 mmol·L^-1 sodium cholata,60 mmol·L^-1 Na₂B₄O₇.The dimersion of the capillary columns was 50 μm×40 cm,30 cm from the inlet to the detection window,on-line UV detector was operated at 200 nm.Results:Under optimum condition,the re- coveries of the analytes in the extact of Radix notoginseng in low,medial,high level was 101.5%,100.6%, 100.4% and the RSD was 2.4%,2.2%,1.0% for ginsengnoside Rg₁,in low,medial,high level was 101.3%, 99.23%,99.66%,and the RSD was 2.0%,1.3%,1.1% for ginsengnoside Re,and in low,medial,high level was 100.6%,100.5%,100.7%,and the RSD was 1.1%,1.1%,0.86% for notoginsengnoside R₁ respectively.Con- clusion:The optimum method puts up a good reproducibility,high accuracy and great linear,which meets the re- quest of analysis.The results show that the method can be used to control quality of Radix et Rhizoma Notoginseng.更多还原