【摘要】 目的:观察紫草多糖对S180荷瘤小鼠瘤重、红细胞膜流动性和带3蛋白含量的影响。方法:采用荧光探针DPH标记法测定红细胞膜的荧光偏振度P,计算微黏度(η)与膜脂流动性(LFU);电泳法测定红细胞膜带3蛋白含量。结果:紫草多糖中剂量组抑瘤率达43.05%;且各剂量组对红细胞膜膜脂流动性、带3蛋白均有影响,以中剂量组效果最佳,与对照组相比P<0.01,P<0.05。结论:紫草多糖通过影响荷瘤小鼠红细胞膜流动性和带3蛋白恢复红细胞的正常功能发挥其抗肿瘤作用。更多还原

【Abstract】 Objective:To observe the influences of polysaccharides of Lithospermum erythrorhizon on fluidity and band-3 protein level of red cells membrance of S180 mice.Method:The fluorescence polarization(P)of red cells was determinend by DPH as probe marking and accounted the microviscosity(η)and membrane fluidity(LFU);the level of band-3 protein was determined by electrophoretic method.Results:Inhibition rate of tumor weight of the middle dose group was arrived at 43.05%,and was influenced the erythrocyte membrane fluidity and band-3 protein level(P<0.01,P<0.05).Conclusion:The antitumour actions of polysaccharides of Lithospermum erythrorhizon may be influenced the erythrocyte membrane fluidity and band-3 protein function to recover cell normal function and resist tumor action.更多还原