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波尔山羊9个微卫星标记与产羔性状的关联研究

Correlation analysis between 9 microsatellite markers and fecundity trait of Boer goat

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【作者】 徐云翔陈世林闻群英沈忠张春艳姚红卫滑国华杨利国

【Author】 XU Yun-xiang1, CHEN Shi-lin1, WEN Qun-Ying2, SHEN Zhong3, ZHANG Chun-Yan1, YAO Hong-Wei1, HUA Guo-Hua1, YANG Li-Guo1 1. Ministry of Education Key Laboratory for Agriculture Animal Heredity and Breeding, Huazhong Agriculture University, Wuhan 430070, China; 2. Shiyan Prologue Bureau, Shiyan 442000, China; 3. Hubei Province Prologue Bureau, Wuhan 430070, China

【机构】 华中农业大学农业动物遗传育种与繁殖教育部重点试验室湖北省十堰市畜牧局湖北省畜牧局华中农业大学农业动物遗传育种与繁殖教育部重点试验室 武汉430070武汉430070十堰442000

【摘要】 选择与绵羊高繁殖力主效基因FecB和FecX紧密连锁的5个微卫星标记BM1329,BM143,OarHH55,TGLA68和LSCV043,和其他4个微卫星标记ETH225,INRA063,BM1225和MAF0214,分析研究其与波尔山羊产羔数的关联。结果表明:所研究的9个波尔山羊微卫星基因座均为高度多态性基因座(PIC>0.5)。找到与波尔山羊产羔性状显著相关的等位基因计12个。其中与波尔山羊第一胎产羔数有显著正效应的等位基因3个:BM143的120bp和108bp,ETH225的183bp;与波尔山羊第一胎产羔数有显著负效应的等位基因8个:BM1329的216bp、BM143的110bp、BM1225的255bp和239bp、INRA063的175bp、177bp和189bp,ETH225的163bp。与波尔山羊第二胎产羔数有显著正效应的等位基因1个:TGLA68的115bp。

【Abstract】 Five microsatellite markers BM1329, BM143, OarHH55, TGLA68, and LSCV043, which were closely linked to the fecundity gene FecB and FecX in ovis aries sheep, were chosen based on their high conservatism in the close species and another 4 microsatellite markers ETH225, INRA063, BM1225, and MAF0214 were selected to analyze their correlation with the litter size of Boer goat. All the 9 microsatellite loci in Boer goat were high polymorphism locus (PIC > 0.5). Twelve alleles were found to be significantly correlate with litter size of Boer goat. Three of them, i.e., 120 bp and 108 bp of BM143, 183 bp of ETH225, were positively correlated to first parity litter size of Boer goat. Eight alleles, i.e., 216 bp of BM1329, 110 bp of BM143, 255 bp and 239 bp of BM1225, 175 bp, 177 bp and 189 bp of INRA063, and 163 bp of ETH225, had negative correlation with first parity litter size. One allele, i.e., 115 bp of TGLA68, were positively correlated to second parity litter size.

【关键词】 波尔山羊微卫星产羔数
【Key words】 Boer goatmicrosatellite markerlitter size
【基金】 国家农业成果转化基金项目(编号:05EFN214200186)资助~~
  • 【分类号】S827
  • 【被引频次】9
  • 【下载频次】183
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