【摘要】 目的探讨氯化镉诱发人支气管上皮细胞系(16HBE)恶性转化过程中hMSH2基因mRNA动态变化的规律。方法用反转录-聚合酶链反应(PT-PCR)技术检测16HBE、氯化镉恶性转化16HBE系不同阶段(第5、15、32代细胞及成瘤细胞)hMSH2基因mRNA的表达情况。结果随着转化代数的增加,hMSH2基因mRNA的表达逐渐降低,到第32代细胞后表达明显下降(P<0.01)。结论氯化镉诱发16HBE系恶性转化过程中hMSH2基因表达逐渐下降,hMSH2基因水平的改变可能是氯化镉的致癌机制之一。更多还原

【Abstract】 Objective To explore the expression of hMSH2 mRNA during the malignant transforming stages of 16HBE cells induced by cadmium chloride.Methods Reverse transcription-polymerase chain reaction(RT-PCR)was used to measure the expression of hMSH2 mRNA in 16HBE cells and its different passage cells treated by cadmium chloride(the 5 th,15 th,32 th passage,and neoplasmed cells from nude mice’s tumor tissue).Results With the passages of 16HBE cells treated with cadmium chloride increased,the expression of hMSH2 gene decreased gradually(P<0.01).Conclusion During transformation from 16HBE cells to malignant transformation induced by cadmium chloride,the expression of hMSH2 gene decreased,the alteration of hMSH2 gene may be a possible carcinogenic mechanism for cadmium chloride.更多还原