【摘要】 目的:探讨急性乙型肝炎、慢性乙型肝炎、HBsAg携带者及正常人血清和PBMC培养上清液中IL-12水平及其意义。方法:应用FicollHypaque密度梯度离心法从肝素化外周血中分离PBMC,用ELISA方法检测血清及PBMC培养上清液IL-12水平。结果:(1)血清IL-12:急性乙型肝炎血清IL-12阳性率30%,慢性乙型肝炎50%,HBsAg携带者10%,正常人为0。(2)PBMC培养上清液IL-12水平:急性乙型肝炎(121.09±97.43)pg/ml,慢性乙型肝炎(57.11±40.51)pg/ml,HBsAg携带者(25.4±15.86)pg/ml,正常人(38.35±33.86)pg/ml。结论:慢性乙型肝炎血清IL-12阳性率显著高于正常人,急性及慢性乙型肝炎PBMC培养上清液IL-12水平显著高于HBsAg携带者,提示HBV感染者血清IL-12阳性率及PBMC培养上清液IL-12水平与肝脏炎症活动有关。更多还原

【Abstract】 Objective:To detect the IL-12 levels of the serum and PBMC culture supernatant in patients with acute hepatitis B,chronic hepatitis B,HBsAg carriers and healthy persons and discuss its significance.Methods:PBMC was isolated by centrifugation on a Ficoll Hypaque density gradient.The IL-12 levels of the serum and PBMC culture supernatant were detected by ELISA method.Results:(1)Serum IL-12:The serum IL-12 positive rate in patients with acute hepatitis B,chronic hepatitis B,HBsAg carriers and healthy persons was 30%,50%,10%,0 respectively.(2)PBMC culture supernatant IL-12:The IL-12 levels of PBMC culture supernatant in patients with acute hepatitis B, chronic hepatitis B,HBsAg carriers and healthy persons were (121.09±97.43)pg/ml,(57.11±40.51)pg/ml,(25.4±15.86)pg/ml,(38.35±33.86)pg/ml respectively.Conclusion:The serum IL-12 positive rate in patients with chronic hepatitis B was significantly higher than that in healthy persons.The IL-12 levels of PBMC culture supernatant in patients with acute hepatitis B and chronic hepatitis B were significantly higher than those in HBsAg carriers.The IL-12 levels of the serum and PBMC culture supernatant have relation to liver inflammation.更多还原