【摘要】 本研究了解真性红细胞增多症(PV)患者血小板膜及骨髓CD34阳性细胞表面TPO受体(c-mpl)蛋白及骨髓造血细胞c-mpl mRNA表达情况。应用双色流式细胞术检测13例PV患者及15名正常对照者骨髓CD34阳性细胞表面c-mpl蛋白表达;应用单色流式细胞术检测15例PV患者及15名正常对照者血小板表面c-mpl蛋白表达;应用RT-PCR方法检查PV患者及正常对照者骨髓造血细胞c-mpl mRNA的表达情况。结果表明:CD34阳性细胞c-mpl蛋白表达在PV患者为0.99%±0.14%,与正常对照(0.92%±0.12%)相比无差别(p>0.05);血小板c-mpl蛋白表达在PV患者为20.33%±4.84%,与正常对照(23.50%±3.64%)相比无差别(p>0.05);骨髓造血细胞c-mpl mRNA与正常组相比也无明显差别(p>0.05。)结论:PV患者骨髓CD34阳性细胞、血小板膜c-mpl蛋白及骨髓造血细胞c-mpl的mRNA表达无明显异常。更多还原

【Abstract】 The objective of this study was to investigate the expressions of TPO receptor (c-mpl) proteins on CD34 positive bone marrow cells (CD34+ BMCs), platelets and the expression of c-mpl mRNA in bone marrow cells of the patients with polycythemia vera (PV). The expressions of c-mpl proteins on CD34+ bone marrow hematopoietic cells of 13 PV patients and 15 normal controls were assessed by bicolor flow cytometry and the expressions of c-mpl proteins on platelets of 15 PV patients and 15 normal controls were assessed by monocolor flow cytometry, and the expressions of c-mpl mRNA in bone marrow hematopoietic cells (BMHCs) were assessed by RT-PCR. The results showed that no difference was found between the expression of c-mpl proteins on CD34+ BMHCs of PV patients(0.99%±0.14%) and that of normal controls (0.92%±0.12%)(p>0.05). There was no difference too between the expression of c-mpl protein on platelets in PV patients(20.33%±4.84%) and that in normal controls (23.50%±3.64%)(p>0.05). No diffe-rence between the expression of c-mpl mRNA in BMHCs of PV patients and that in normal controls was seen. In conclusion, the expressions of c-mpl proteins on CD34+ BMHCs, platelets and c-mpl mRNA in BMHCs of PV patients were not obviously abnormal.更多还原