【摘要】 利用Modeller7v7软件对米根霉(Rhizopus oryzae)富马酸酶(fumarase)进行了三级结构的同源建模并对结果的空间和能量上的合理性进行了验证,进一步对酶的结构域和催化活性位点进行了研究。结果表明:富马酸酶由三个结构域组成,中心区域为一个由五个几乎平行的α螺旋组成的独特的束型结构,其催化活性位点是由三个亚基上的氨基酸相互靠近共同组成的。为以后有针对性的进行富马酸酶的定点突变提高富马酸产量提供分子水平上的理论指导。更多还原

【Abstract】 Homology three-dimensional structure modeling of fumarase from Rhizopus oryzae was conducted by bioinformatics software Mode- ller7v7 and the reliability of the results was proved,the domain and active sites of fumarase was studied based on the results.The results show that fumarase from Rhizopus oryzae in this study consists of three domains,and the core domain is composed of 5 nearly parallelα-helixes. The active amino acids of fumarase are formed by amino acids from three different subunits.This experiment will help us to better understand the catalytic mechanism of fumarase and make the site-specific mutagenesis to improve the production of fumaric acid in future.更多还原