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罗汉果试管苗瓶外生根研究

Studies on the In vitro and Ex vitro rooting for test-tube seedlings of Momordica grosvenorii

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【作者】 黄卓忠; 严华兵; 苏国秀;

【Author】 HUANG Zhuo-Zhong,YAN Hua-Bing,SU Guo-Xiu (Bio-technology Research Institute,Guangxi Academy of Agricultural Sciences,Guangxi Nanning 53007,China)

【机构】 广西农业科学院生物技术研究所; 广西农业科学院生物技术研究所 广西南宁530007; 广西南宁530007;

【摘要】 以罗汉果试管苗为材料,采用非试管快繁智能化育苗大棚进行了瓶内和瓶外生根移栽试验。结果表明,基部不带愈伤组织的试管苗瓶外生根处理后,生根率、最长根长、生根数分别为96.11%、4.53 cm、3.6条,最长根长显著高于瓶内生根处理,而生根率和生根数两者差异不显著;瓶内生根苗移栽成活率显著高于瓶外生根苗。通过缩短瓶外生根时间,增施叶面肥等措施,可获得较高的移栽成活率。由此证明了采用试管苗瓶外生根技术可大大提高罗汉果试管苗的生根质量。

【Abstract】 Ex vitro and In vitro rooting and transplanting experiments were carried out by taking test-tube seedlings of Momordica grosvenorii as explants.The results showed that in the treatment of ex vitro rooting by using the seedling without callus,the rooting percentage,the length of longest root and the number of roots are 96.11%,4.53 cm,3.6 respectively.The length of longest root is significantly higher than that of in vitro rooting but the rooting percentage and the number of roots show no great difference.The plantlets in vitro rooting had higher survival rate than that of ex vitro rooting.Meanwhile,the technology of ex-vitro rooting for test-tube plantlets could improve rooting quality greatly.

【基金】 广西农科院科技发展基金“植物非试管快繁技术在紧缺药用植物上的研究与应用”(2006022)
  • 【文献出处】 西南农业学报 ,Southwest China Journal of Agricultural Sciences , 编辑部邮箱 ,2007年04期
  • 【分类号】S668.9
  • 【被引频次】21
  • 【下载频次】211
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