【摘要】 采用酚/氯仿少量提取法、异丙醇沉淀法、简便快速的煮沸法以及微波炉法提取细菌质粒,然后分别对各种方法所提取的质粒进行琼脂凝胶电泳分析,并用紫外分光光度计测定其OD值,对各种方法的优劣性进行全面分析,结果表明异丙醇提取法所提取的质粒DNA纯度和产量高(其DNA片段荧光较强,具有清晰的亮带,OD260/OD280约为1.77,DNA样品浓度约为195μg/mL),且重复性好,具有结果稳定的特点,达到了分子生物学试验要求,适合大多数试验室使用。更多还原

【Abstract】 In this experimental study,phenol-chloroform extraction,isopropyl alcohol extraction,simple and quick boil,and microwave heating method were used to extract plasmid.Then agarose gel electrophoresis was used to analyze the respective plasmid DNA obtained by different methods.Finally,OD values were measured by the method of ultraviolet spectrophotometer determination and each method’s advantages and disadvantages were analyzed.High yield with higher purity and better repetition was obtained in the second method(the fragment of DNA had strong fluorescence and clear bright band,OD260/OD280 was 1.77,the density of DNA sample was 195 μg/mL approximately),the results also showed that this method displayed better reproducibility and stronger stability,which may be used for molecular biology experimentation.It was concluded that isopropyl alcohol extraction method was very suitable to be used by most laboratories.更多还原