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大花蕙兰工厂化繁殖技术研究
The study on factory propagation technique of Cymbidium grandiflorium
【摘要】 研究了大花蕙兰原球茎诱导技术,快繁增殖技术,同步化发育技术以及生根苗假植技术。结果表明:①适当浓度的6-BA使各参试品种都能产生原球茎,3个品种平均诱导率约为64%,其中1#、2#、3#品种的最佳诱导率分别为80%、47%、64%。②较低浓度6-BA与较低浓度的NAA配合,可使各参试品种原球茎30天的平均增殖率达到4~5倍,个别增殖达10倍以上。③通过综合调控可以使90%以上的原球茎按照期望的方向同步发育。
【Abstract】 In this paper,we studied the propagation techniques of cymbidium grandiflorium on inducing protocorm-like bodies(PLBs);high-speed propagation of PLBs;development homogenization of PLBs and false plant of rooting seedling.The results showed that :(i)PLBs were normally formed by suitable concentration 6-BA,and average inducing ratio of three varietys was 64 %,the best inducing ratio of 1#,2 # and 3# variety were respectively 80 %,47 % and 64 %;(ii)As concentration of 6-BA and NAA in medium was lower samely,the all experimental variety average propagation ratio of PLBs can arrived 4-5 times or 10 times in 30 days;(iii)More than 90 % PLBs can synchronize as required development orientation by synthetical step of adjustment and control.
【Key words】 Cymbidium grandiflorium; protocorm-like bodies(PLBs); fast propagation; homogenization development;
- 【文献出处】 西南农业学报 ,Southwest China Journal of Agricultural Sciences , 编辑部邮箱 ,2007年04期
- 【分类号】S682.31
- 【被引频次】12
- 【下载频次】112