【摘要】 目的探讨槐定碱(Sophojridine,SRI)致痫大鼠海马组织中谷氨酸(Glu)和γ-氨基丁酸(GABA),血清中肿瘤坏死因子-α(TNF-α)、白细胞介素-2(IL-2)和白细胞介素-6(IL-6)的变化。方法腹腔注射槐定碱(47.83mg/kg)诱导大鼠癫痫,采用放射免疫测定法检测TNF-α、IL-2和IL-6表达的水平,免疫组化法测定Glu和GABA阳性神经元的含量。结果模型组大鼠血清中TNF-α、IL-2和IL-6含量均明显高于对照组(P<0.01),大鼠海马组织中Glu免疫反应阳性细胞数,模型组均明显高于对照组,GABA免疫反应阳性细胞数,模型组均明显低于对照组(P<0.05)。结论TNF-α、IL-2、IL-6可能参与了癫痫的发病;Glu和GABA等氨基酸类神经递质平衡紊乱可能是癫痫发病的基础之一。更多还原

【Abstract】 Objective To investigate the changes of the contents of glutamate(Glu)、 gama-aminobutyric(GABA),serum IL-2,IL-6 and TNF-α in epileptic rats induced by sophoridien(SRI).Methods We establish epileptic rats model induced by SRI with intraperitoneal injection.The levels of serum TNF-α,IL-2 and IL-6 of each group were assayed by radioimmuoassay(RIA).Meanwhile we measured the contents of glutamate(Glu) and gama-aminobutyric(GABA) of the hippocampus by immunohistochemistry.Results The levels of serum TNF-α,IL-2 and IL-6 were significantly higher in model group than those in control group(P<0.01).Glu positive cells in hippocampus in model group were more than those in control group.Furthermore,GABA positive cells in model group were less than those in control group(P<0.05).Conclusion TNF-α,IL-2 and IL-6 may be involved in the pathogenesis of epilepsy.Neurotransmitter balance disorderin of the Glu and GABA may be the base of pathogenesis of epilepsy.更多还原