【摘要】 设计3对引物,分别扩增链球菌属特异性gdh、猪链球菌种特异性16S rRNA和猪链球菌2型特异性cps2J等基因,目的片段大小分别为725bp、523bp和387bp。利用合成的3对引物并通过对反应条件与反应体系的优化建立了多重PCR。应用该多重PCR检测了分离到的链球菌1105株,检出猪链球菌667株,猪链球菌2型33株。研究结果表明,该方法特异性高、敏感性强,可广泛应用于猪链球菌病的快速诊断及流行病学调查。更多还原

【Abstract】 Three pairs of primers were designed to amplify three genes, Streptococci genus specific gene (gdh), Streptococci suis species specific gene(16S rDNA)and Streptococci suis type 2 gene(cps2J). The PCR products were 725 bp, 523 bp and 387 bp, respectively. A multiplex PCR was established by optimizing the reaction conditions and used to detect 1 179 samples. The result showed that there were 667 strains of S. suis and 33 strains of S. suis type 2 in 1 105 strains of Streptococcus detected. A sensitive and specific multiplex PCR assay was established for the application in diagnosis and epidemiological survey of S. suis infection.更多还原