【摘要】 采用高效液相色谱与质谱联用法简单快速测定猪肉组织中8种头孢类抗生素的残留。样品添加8种头孢类抗生素（头孢羟氨苄、头孢氨苄、头孢克洛、头孢拉定、头孢噻肟、头孢唑啉、头孢孟多酯钠、头孢噻吩）,采用0.5%的冰醋酸水溶液提取,匀浆离心分离后通过固相萃取柱净化,甲醇洗脱,用二极管阵列检测器及质谱检测器检测,信噪比较高,数据结果准确。采用XTerra MS-C₁₈（2.1×150mm,5μm）色谱柱,以10mmoL/L甲酸铵和乙腈为流动相进行梯度洗脱。对于不同的药物,回收率范围为52%～98%（添加水平为0.1mg/kg,0.2mg/kg,0.5mg/kg）,相对标准偏差在1.1%～10.55%以内,方法的最低检出限为0.2ppb～2.2ppb。大量的实验结果表明,该方法适合于猪肉中头孢类抗生素残留的测定。更多还原

【Abstract】 A simple and rapid method was developed for the determination of 8 Cephalosporins residues in pork by high performance liquid chromatography tandem mass spectrometry.The samples,with spiked 8 Cephalo- sporins（ Cephadroxi、Cephalexin、Cephaclor、Cepharadine、Cephotaxime、Cephazolin、Cephamandole Sodium Salt、Cephalothin） were extracte 0.5% glacial acetic acid,followed by homogenizing and centrifuging precipitation, purified with solid-phase extraction column,eluted by methanol.The separation was performed on a XTerra MS -C₁₈ （2.1×150mm,5μm）column using a mobile phase of 10mmol/L ammonium formate and acetonitrile and de- tected with diode-array detector,S/N is higher,data results is veracity.The limit of detection of the method is 0.2～2.2ppb for the various antibiotics and the recovery of the spike experiment is from 52% to 98% at three differ- ent spiked levels 0.1mg/kg,0.2mg/kg,0.5mg/kg,relative standard deviations are between 1.1%～10.55%.The results showed that the method is suitable for the determination of residues of Cephalosporins in pork.更多还原