【摘要】 目的探讨环孢霉素A(cyclosporine A,CsA)对胰岛β细胞(NIT-1细胞)胰岛素分泌及细胞凋亡的影响。方法实验室培养NIT-1细胞,用0.5,2.5,5.0,10μmol.L-1CsA作用细胞72 h,检测其对细胞胰岛素分泌及凋亡的影响。结果与对照组相比,CsA能明显抑制NIT-1细胞胰岛素分泌(P<0.01),对细胞的凋亡具有明显促进作用(P<0.01)。结论CsA对NIT-1细胞的功能具有抑制作用并能促进细胞的凋亡。更多还原

【Abstract】 OBJECTIVE To explore the effect of cyclosporine A on pancreatic β cells(NIT-1) insulin secretion and apoptosis.METHODS Cultured pancreatic β cells(NIT-1) and detected the effect of cell insulin secretion and apoptosis after 0.5,2.5,5.0,10 μmol·L-1 CsA affected cells for 72 hours. RESULTS Compared with controls,CsA could significantly inhibit NIT-1 insulin secretion(P<0.01) and significantly promote apoptosis(P<0.01).CONCLUSION CsA can inhibit NIT-1 function and promote apoptosis.更多还原