【摘要】 目的:观察活血降糖饮对糖尿病大鼠血糖、血脂、血浆胰岛素、胰高糖素、胰岛素敏感性指数及氧自由基等指标的干预作用。方法:实验于2001-03/08在广州中医药大学第一临床医学院糖尿病研究所实验室进行。①造模:取80只SD大鼠随机分为2组,一组65只腹腔注射链脲佐菌素诱发糖尿病,另一组15只腹腔注射柠檬酸缓冲液为正常对照组。②给药:将造模成功的56只大鼠随机分为模型空白组、玉泉丸组、优降糖组及活血降糖饮组4组,每组14只。模型空白组每日灌服3mL生理盐水,玉泉丸组每日灌服玉泉丸混悬液3.75g/kg,优降糖组每日灌服优降糖混悬液1.25mg/kg,活血降糖饮组每日灌服活血降糖饮水煎液(黄芪30g、生地20g,丹参30g,太子参30g,五味子15g,麦冬15g,淮山12g,黄精15g,丹皮12g,大黄16g,川红花12g,桃仁10g组成)0.01mL/g,治疗5周。③评估指标:5周后采血检测空腹血糖、血脂、过氧化脂质、超氧化物歧化酶、血浆胰岛素、胰高糖素,并计算胰岛素敏感性指数。结果:60只大鼠进入结果分析。①空腹血糖:造模后各组均较造模前升高(P<0.001),治疗后活血降糖饮组低于玉泉丸组,高于优降糖组[(13.22±6.88),(15.35±7.23),(10.28±6.75)mmol/L,P<0.05],3组均高于正常对照组。②血脂:活血降糖饮组和玉泉丸组三酰甘油及胆固醇低于优降糖组,高密度脂蛋白胆固醇高于优降糖组(P<0.05)。③血浆胰岛素:治疗后优降糖组高于造模后[(9.15±2.66),(4.61±1.55)mU/L,P<0.05],玉泉丸组与活血降糖饮组与造模后无差异。④胰高糖素:治疗后各组均明显下降(P<0.01),其中活血降糖饮组下降程度强于玉泉丸组,弱于优降糖组(P<0.05)。⑤胰岛素敏感性指数:治疗后玉泉丸组与活血降糖饮组均较造模后显著改善(P<0.01),优降糖组无明显变化。⑥治疗后,治疗各组血浆过氧化脂质水平降低,超氧化物歧化酶活性升高,与空白组比较差异显著(P<0.01),但仍未达到正常对照组水平(P<0.05)。结论:活血降糖饮对实验性糖尿病大鼠有降低空腹血糖、改善血脂、降低胰高糖素及增强胰岛素敏感性的作用。更多还原

【Abstract】 AIM:To observe the interventional effects of Huoxue jiangtang yin on blood glucose,blood lipids,plasma insulin,glucagon,insulin sensitivity index and oxygen free radicals of experimental diabetes rats. METHODS: The experiment was undertaken at laboratory of Institute of Diabetes,First Clinical Medical College,Guangzhou University of Chinese Medicine from March to August 2001. ① Totally 80 SD rats were divided into 2 groups randomly: 65 rats in group A were treated with streptozotocin (STZ)-induced diabetes by intraperitoneally; 15 in group B were treated with citrate buffer solution by intraperitoneally as normal control group. ② The 56 rats were divided into 4 groups randomly: model blank group,Yuquanwan group,glybenclamide group and Huoxue jiangtang yin group with 14 in each group. Rats in the model blank group were given 3 mL saline by gastric perfusion every day. Rats of the Yuquanwan group were drenched suspension of Yuquanwan (3.75 g/kg) each day. Rats of the glybenclamide group were drenched suspension of glybenclamide (1.25 mg/kg) each day. Rats of the Huoxue jiangtang yin group were drenched liquid of Huoxue jiangtang yin (composed of 30 g astragalus mongholicus,20 g rehmannia dride rhizome,30 g salvia miltiorrhiza,30 g heterophylly falsestarwort root,15 g fructus schizandrae,15 g ophiopogonis tuber,12 g dioscorea batatas,15 g solomonseal rhizome,12 g cortex moutan,16 g rhubarb,12 g sichuan safflower and 10 g peach seed; 0.01 mL/g) for 5 weeks. ③ After 5-week treatment,fasting blood glucose,blood lipids,lipid peroxidation (LPO),superoxide dismutase (SOD),plasma insulin and glucagon were measured and the insulin sensitivity index was calculated. RESULTS: Totally 60 rats were involved in the result analysis. ① The level of fasting blood glucose was increased after establishing models (P < 0.001). It was lower in the Huoxue jiangtang yin group than the Yuquanwan group,but higher than the glybenclamide group (13.22±6.88),(15.35±7.23),(10.28±6.75) mmol/L,P < 0.05. The levels in the three groups were all higher than that in the normal control group. ② The levels of triglyceride and cholesterin in the Huoxue jiangtang yin group and Yuquanwan group were lower than that in the glybenclamide group. Level of high-density lipoprotein cholesterol was higher than that in the glybenclamide group (P < 0.05). ③Plasma insulin was higher in the glybenclamide group after treatment as compared with that after establishing models (9.15±2.66),(4.61±1.55) mU/L,P < 0.05. There was no significant difference between Yuquanwan group and Huoxue jiangtang yin group after establishing models. ④Glucagon was decreased obviously after treatment in each group (P < 0.01). The reduction of Huoxue jiangtang yin group was higher than that in the Yuquanwan group,but lower than that in the glybenclamide group (P < 0.05). ⑤Insulin sensitivity index was ameliorated markedly in the Yuquanwan group and the Huoxue jiangtang yin group after treatment as compared with that after establishing models (P < 0.01). There was no significant change in the glybenclamide group. ⑥After treatment,level of plasma LPO was decreased but level of SOD was increased. There were significant differences as compared with the blank group (P < 0.01),but did not reach the level in the normal control group (P < 0.05). CONCLUSION: Huoxue jiangtang yin may bring down fasting blood glucose and glucagon,ameliorate blood lipids,and improve the insulin sensitivity in experimental diabetes rats.更多还原