【摘要】 目的:观察早期限饲(出雏后两周隔天饲喂)对肉鸡脂质过氧化作用和抗氧化酶活性产生的长期影响,并通过与后期限饲(屠宰前两周隔天饲喂)比较,观察不同阶段限饲对肉鸡血清、肝脏、胸肌、腓肠肌丙二醛浓度、超氧化物歧化酶和谷胱甘肽过氧化物酶活性的影响。方法:实验主要于2005-04/12在南京农业大学农业部动物生理生化重点开放实验室完成。实验分组:选取1日龄健康快三黄商品肉鸡100羽随机分为2组,对照组60羽,早期限饲组40羽。饲养至50日龄,从对照组随机选取20羽作为后期限饲组。实验处理:①早期限饲组,1～14日龄进行隔日限饲,以后自由采食。②后期限饲组,1～49日龄自由采食,50～63日龄进行隔日限饲。③对照组,全程自由采食。实验评估:记录每周体质量,检测14日龄对照组、早期限饲组,63日龄对照组、早期限饲组和后期限饲组血清、肝脏、胸肌、腓肠肌丙二醛浓度、超氧化物歧化酶和谷胱甘肽过氧化物酶活性。结果:100羽实验动物均进入结果分析。①14日龄早期限饲组肉鸡血清、肝脏、胸肌和腓肠肌丙二醛浓度、超氧化物歧化酶和谷胱甘肽过氧化物酶活性与对照组相比差异无显著性。②63日龄时早期限饲组血清丙二醛浓度和谷胱甘肽过氧化物酶活性均显著高于对照组(P<0.05),肝脏超氧化物歧化酶活性显著低于对照组(P<0.05);后期限饲组血清丙二醛浓度以及超氧化物歧化酶和谷胱甘肽过氧化物酶活性均显著高于对照组(P<0.05),而肝脏丙二醛浓度、胸肌超氧化物歧化酶活性显著低于对照组(P<0.05),腓肠肌各项指标与对照组相比差异无显著性。后期限饲组血清谷胱甘肽过氧化物酶活性显著高于早期限饲组(P<0.05)。后期限饲组肝脏丙二醛浓度显著低于早期限饲组,超氧化物歧化酶活性显著高于早期限饲组(P<0.05)。结论:早期及后期限饲均能增强63日龄肉鸡体内整体水平脂质过氧化作用和抗氧化酶活性,早期限饲对肉鸡脂质过氧化作用和血清抗氧化酶活性的即时影响表现不明显,但其影响可以持续到后期。更多还原

【Abstract】 AIM:To investigate the long-term effect of early post-hatch feed restriction (EFR,food provided every other day during first 2 weeks after hatching) on the lipid peroxidation and enzymatic antioxidant activity in broiler chicken,compare with the effect of late feed restriction (LFR,performed 2 weeks before slaughtering),and observe the changes of the concentrations of malonilaldehyde (MDA),activities of superoxide dismutase (SOD) and glutathione peroxidase (GPX) in plasma,liver,pectoral and gastrocnemius muscle. METHODS: The experiment was conducted at Key Laboratory of Animal Physiology and Biochemistry,State Ministry of Agriculture,Nanjing Agricultural University from April to December in 2005. Experimental grouping: Totally 100 healthy chicken of one-day old were selected and randomly assigned into two groups: control group (n =60) and EFR group (n =40). Controlled chicken were fed to 50-day old,and 20 of them were selected as LFR group. Experimental management: ①EFR group: Feed restriction was performed every other day during first 2 weeks,followed by free feeding.②LFR group: Free feeding at days 1-49 and feed restriction at days 50-63 were combined.③Control group: Free feeding was managed only. Experimental evaluation: Body weight was recorded every week. At days 14 and 63,the concentrations of MDA,activities of SOD and GPX in plasma,liver and muscle were measured. RESULTS: All 100 animals were involved in the result analysis.①No significant changes were found for all the indices at 14 days of age between EFR group and control group.②At 63 days of age,EFR chicken exhibited significantly higher serum MDA concentration and GPX activity compared with the control counterparts (P < 0.05),significantly lower liver SOD activity (P < 0.05). LFR chicken also showed significantly higher serum MDA concentration as well as SOD and GPX activities at 63 days of age,but significantly lower liver MDA concentration and pectoral muscle SOD activity compared with the control counterparts (P < 0.05). No change was observed in gastrocnemius muscle (P < 0.05). Compared with EFR group,serum GPX and SOD activities were increased,but liver MDA concentration was decreased in LFR group (P < 0.05). CONCLUSION: Both EFR and LFR can increase lipid peroxidation and enzymatic antioxidant activity systematically in broiler chicken of 63 days old. EFR exerts long-term effect on lipid peroxidation and enzymatic antioxidant activity.更多还原