【摘要】 目的:探讨天参益智复方对β淀粉样蛋白(β-amyloid peptide,Aβ)引起的神经母细胞瘤SH-SY5Y细胞尼古丁受体(nicotinic acetylcholine receptor,nAChR)亚单位在蛋白质和mRNA水平降低、细胞损伤及脂质过氧化的拮抗作用。方法:选取一定浓度的天参益智复方制剂预处理SH-SY5Y细胞后,再加入Aβ蛋白处理,比色法测定细胞甲基噻唑基四唑还原率,从细胞整体水平了解此复方制剂对细胞活性的影响。蛋白质印迹法和逆转录聚合酶链法分别从蛋白质水平和mRNA水平测定nAChR亚单位α3、α7表达的变化。硫代巴比妥酸比色法测定脂质过氧化产物丙二醛的水平,以了解该复方制剂的抗氧化能力。结果:安全浓度的天参益智复方对α7的蛋白质表达水平有上调作用;能对抗Aβ引起的α3和α7 nAChR亚单位蛋白质水平降低及α7亚单位mRNA表达低下,但对α3亚单位mRNA水平无明显影响;能减弱Aβ引起的细胞损伤和脂质过氧化水平升高。结论:天参益智复方制剂能在蛋白质水平上调尼古丁受体α7亚单位,对抗Aβ引起的nAChR表达降低及神经毒性作用,在阿尔茨海默病的治疗中可能起到一定作用。更多还原

【Abstract】 Objective:To investigate the inhibition effects of Tianshen Yizhi Recipe（TSYZR）,a compound traditional Chinese herbal medicine,on decreased expression of nicotinic acetylcholine receptor（nAChR）and the neurotoxicity as well as lipid peroxidation induced by beta-amyloid peptide（Aβ）in human SH-SYSY neuroblastoma cells. Methods:The SH-SY5Y cells were treated by a certain concentration of TSYZR,and then exposed to Aβ_25-35, Methyl thiazolyl tetrazolium reduction assay was carried out to understand the influences of the drugs on cellular viability.Expressions of nAChR subunits（α3 andα7）at protein and mRNA levels were detected by Western-blotting and reverse transcription polymerase chain reaction,respectively.Lipid peroxidation was measured by thiobarbituric acid to observe the capacity of antioxidant of the drugs. Results:TSYZR at a safe concentration could increaseα7 protein in the cells,inhibit decreased expressions ofα3 andα7 nAChR subunit proteins,prevent lower expression ofα7 mRNA in SH-SY5Y cells induced by Aβ, reduce the neurotoxicity and lipid peroxidation resulting from Aβ,but had no significant effect on the lower expression ofα3 mRNA. Conclusions:TSYZR can up-regulate the expression ofα7 nAChR subunit protein and prevent decreased expressions of nAChRs and neurotoxicity as well as lipid peroxidation induced by Aβ.This drug may play an important therapeutic role in treatment of Alzheimer disease.更多还原