【摘要】 目的优化制备更昔洛韦脂质体的处方。方法采用逆相蒸发法制备脂质体,在单因素实验的基础上,以包封率和泄漏率为指标综合评分,采用正交设计实验的方法优化制备更昔洛韦脂质体的处方。结果更昔洛韦脂质体的最佳制备处方为:药物∶(磷脂+胆固醇)为1∶12(质量比),胆固醇与卵磷脂质量比为1∶5,吐温-80∶(磷脂+胆固醇)比为1∶15(质量比),有机相与水相体积比为1∶2.5,在60℃时采用逆向蒸发法制备,制得的更昔洛韦脂质体包封率达43.24%,泄露率为1.02%,粒径为332.3nm。结论按最优制备处方制得的更昔洛韦脂质体的包封率较高,泄漏率较低。更多还原

【Abstract】 Objective To optimize the formulation of ganciclovir liposome. Methods Orthogonal design was adopted to screen the formulation of ganciclovir liposome on the basis of the score of the entrapment efficiency and leaking percentage. Results The optimization conditions are drug∶lipid=1∶12, cholesterol∶lecithin=1∶5, tween-80∶lipid=1∶15, chloroform:water=1∶2.5. The entrapment efficiency and leaking percentage of liposome, which was prepared on the optimization conditions, are 43.24% and 1.02%, respectively,and the particle size of the liposome was 332.3nm. Conclusion The entrapment efficiency of the optimized ganciclovir liposome and the leaking percentage are desirable.更多还原