【摘要】 研究了前体物质和碳氮源补加策略对钝齿棒杆菌Corynebacterium crenatum SYAN7发酵生产L-精氨酸的影响。结果表明,发酵24h后添加前体物质谷氨酸,以初始硫酸铵质量浓度20 g/L,分别于24、48h补加20 g/L硫酸铵的补氮方式,和以初始葡萄糖质量浓度100 g/L,分别于24、48、72h均匀添加葡萄糖,使总糖质量浓度达175 g/L的补碳方式,摇瓶发酵96 h后,L-精氨酸产量最高达37.8 g/L。更多还原

【Abstract】 The effect of precursor and C & N source feeding strategy on L-Arginine production by Corynebacterium crenatum SYAN7 was studied.The results showed that the yield of L-Arginine was increased to 37.8 g/L by the combination of the feeding glutamic acid after 24 h,the feeding （NH₄）₂SO₄ in 24 h （20 g/L） and 48 h （20 g/L） with the initial （NH₄）₂SO₄ concentration 20 g/ L,the feeding glucose in 24 h,48 h and 72 h till the total glucose 175 g/L with the initial glucose concentration 100 g/L.更多还原