【摘要】 在对面包酵母生物合成ATP的影响因素研究的基础上,初步探讨了生物合成ATP的反应机理。研究结果表明:在反应体系中直接添加终体积分数为0.5%的甲苯,可显著提高面包酵母细胞膜通透性。Mg²⁺对ATP的合成影响很大,当Mg²⁺浓度为40 mmol/L时,ATP合成量达到最大值,为9.02 mmol/L。在面包酵母生物合成ATP的过程中,当葡萄糖磷酸化和腺苷磷酸化反应竞争时,葡萄糖磷酸化快于腺苷磷酸化,在体系中其它需能反应结束后ATP才开始大量积累。在面包酵母经糖酵解途径生物合成ATP的前期,NAD的再生主要通过形成甘油和乙醇来实现,而在生物合成ATP的中期和快速合成期,NAD的再生主要是通过形成乙醇来实现。更多还原

【Abstract】 Based on the study of factors of ATP biosynthesis in Saccharomyces cerevisiae WSH2, the mechanism of ATP biosynthesis was investigated.The results showed that toluene direct treatment with the final concentration of 0.50%(v/v) was beneficial for the improvement of penetrability of cell membrane.Magnesium ion greatly affected the synthesis of ATP,and the highest ATP production(9.02 mmol/L) could be achieved at 40 mmol/L.It was found that the rate of glucose phosphorylation was faster than that of adenosine phosphorylation.ATP was accumulated rapidly only in the final phase.In case of ATP biosynthesis,NAD regeneration was mainly conducted via the formation of glycerol and ethanol.While in metaphase and fast synthesis phase of ATP,NAD regeneration was mainly conducted by the formation of ethanol.更多还原