【摘要】 考察了不同pH值(4.0、7.0、9.0)条件下采用不同截留相对分子质量(MWCO)的8000复合膜(醋酸纤维+聚砜)和5000 PES(聚醚砜)的超滤膜对大豆肽处理前后对小鼠体外免疫功能的影响。通过体外培养白细胞实验,比较了大豆肽超滤处理前后对小鼠腹腔巨噬细胞吞噬功能和脾细胞增殖能力的影响。结果表明,所有大豆肽均能提高小鼠腹腔巨噬细胞的吞噬能力,促进小鼠脾淋巴细胞增殖,而经过超滤处理后的大豆肽免疫功能有所提高,且以pH4.0条件下MWCO为5000超滤膜处理后所得的大豆肽活性最强。更多还原

【Abstract】 In this paper,the effects of soybean peptide treated by fore-and-aft ultrafiltration with MWCO 8 000 complex membrane and 5 000 PES membrane under different pH(4.0,7.0,9.0) on immune functions of mice in vitro were investi gated.Neutral red and MTT were used to determined the effects of different peptides on phagocytosis of mouse peritoneal macrophage and proliferation of mouse splenic lymphocytes.All of the peptides improved quadratically the phagocytic index of macrophage and the stimulation index of splenic lymphocytes significantly and after treated by ultrafiltration,peptides’ immune function were higher than that of before.The results indicated that,all of the peptides could enhance immune function in mice;ultrafiltration treatment on peptide can improve the immune function of peptide effectively and the peptide which was treated by MWCO 5 000 under pH4.0 has the highest activity.更多还原