【摘要】 利用WL营养琼脂对采自葡萄园和葡萄汁发酵过程中的35株酵母菌进行了分类鉴定,同时进行了5.8S-ITS和26S rDNA D1/D2区的扩增与测序。结果表明利用WL营养琼脂的鉴定结果与测序结果基本符合。WL营养琼脂是一种较为有效的葡萄酒相关酵母菌的分类鉴定培养基。更多还原

【Abstract】 35 yeasts colonies isolated from vineyards and fermentation were plated onto WL nutrient agar to evaluate colony diversity.Sequence analysis of 5.8S-ITS and large ribosomal 26S rRNA indicate that different yeast population can be identified on the basis of unique colony morphology with WL nutrient agar thus validates the effectiveness of using WL nutrient agar as classification and identification culture medium.更多还原