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复合诱变和抗性筛选利迪链菌素高产菌株
Breeding of High Streptolydigin-Producing Strain by Complex Mutagenesis and Resistance Selection
【摘要】 为解决利迪链霉菌生产能力低的问题,以Streptomyces lydicus AS4.2501-P28为出发菌株,应用紫外和吖啶橙诱变,结合抗生素的抗性筛选,得到稳定高产的S.lydicus AS4.2501-L8,发酵水平达到177.0μg/mL,较出发菌株提高了96.2%。
【Abstract】 A high Streptolydigin-producing strain Streptomyces lydicus AS 4.2501-L8 with genetic stability was obtained from the original strain S. lydicus AS 4.2501-P28 by mutagenesis with UV and acridine orange, followed by resistance selection. Its yield of Streptolydigin was 177.0 μg/mL, 96.2% higher than the original strain.
【关键词】 利迪链霉菌;
利迪链菌素;
复合诱变;
抗性筛选;
【Key words】 Streptomyces lydicus; Streptolydigin; Complex mutagenesis; Resistance selection;
【Key words】 Streptomyces lydicus; Streptolydigin; Complex mutagenesis; Resistance selection;
【基金】 国家自然科学基金资助项目(No20425620)
- 【文献出处】 微生物学通报 ,Microbiology , 编辑部邮箱 ,2007年05期
- 【分类号】Q933
- 【被引频次】18
- 【下载频次】292