【摘要】 目的探讨肠易激综合征(irritable bowel syndrome,IBS)患者结肠黏膜中褪黑素受体(melatonin receptor,MR)的表达情况及其与肠易激综合征临床症状的相关性。方法用免疫组化法,检测正常对照20例、腹泻型肠易激综合征(diarrhea-predominantIBS,D-IBS)23例、便秘型肠易激综合征(constipation-predominant IBS,C-IBS)20例结肠黏膜中MR的表达情况。使用JEDA801D形态学图像分析系统,测量阳性表达面积及光密度(opacity density,OD)。结果MR在黏膜层含量较多,黏膜下层含量较少。回盲部和乙状结肠MR阳性面积及OD值表现为:D-IBS明显高于正常对照组(P<0.05),正常对照组明显高于C-IBS(P<0.05)。与同组IBS乙状结肠相比,回盲部阳性面积增高(P<0.05);OD值无明显差异。结论MR在D-IBS中高表达,在C-IBS中低表达,说明MR对IBS患者胃肠道运动有一定影响。更多还原

【Abstract】 Objective To investigate expression of melatonin receptor(MR) in colon mucosa from IBS and its the clinical significance.Methods Twenty three diarrhea-predominant IBS(D-IBS),20 constipationpredominant IBS(C-IBS)(according to Rome Ⅱ) and 20 control participated in the protocol.The expression of MR in ileocecal junction(ICJ) and the sigmoid colon of patients were texplored by Streptavidi/Peroxidase(SP) immunohistochemistry.The pathology microscope image analysis system(JEDA801D) was applied for the positive area and the optical density(OD) scale.Results There were more MR in mucous layer than submucous layer.Significant increase of MR positive area and OD scale were found in the ileocecal junction and the sigmoid colon biopsies from D-IBS patients compared to controls(P<0.05) and from controls than C-IBS(P<0.05).MR positive area in the ileocecal junction from IBS patients showed a significant increase comparing to that in the sigmoid colon from the same group(P<0.05),but the difference of OD scale between the ileocecal junction and the sigmoid colon biopsies from the same IBS group was not significant(P>0.05).Conclusions The expression of MR is high in D-IBS and is low in C-IBS.This suggests that MR has some influence to gastrointestinal motility.更多还原