【摘要】 采用酶学方法测定了盐度(5、15、25、35)胁迫下锯缘青蟹血清中酚氧化酶(PO)及其肌肉和肝胰腺中超氧化物歧化酶(SOD)活性的变化.结果表明,盐度胁迫显著影响青蟹血清PO活性(p<0.05).S5、S15低盐度处理组青蟹血清中PO活性显著高于S25、S35高盐度处理组(p<0.05),同一处理组青蟹血清中PO活性除S15处理组随胁迫时间延长而持续下降外,其余各组呈现出有升有降的变化.各处理组在盐度低于或高于25时,青蟹肌肉中SOD活性升高,且胁迫至72h前,随着胁迫时间延长,SOD活性下降;青蟹肝胰腺中SOD活性随着盐度升高而降低,且同一处理组青蟹肝胰腺中SOD活性则随着胁迫时间的延长而呈现出先降后升的趋势.这表明环境盐度胁迫对青蟹体内SOD活性影响显著,且表现出明显的时间效应性.由此可见,盐度胁迫显著影响青蟹的PO、SOD活性,进而影响青蟹免疫力.更多还原

【Abstract】 This paper studied the effect of salinity(5,15,25 and 35) stress on the biochemical response of Scylla serrata by enzyme analysising.Scylla serrata were acclimated in close-cycle system before transferred to different groups.Each treatment was run in triplicate.The activities of phenoloxidase(PO) and superoxide dismutase(SOD) were determined after 24~96h.The results showed that the phenoloxidase activity was increased significantly with salinity decreasing in salinty(p<0.05).The serum PO activities of S5 and S15 were significantly higher than those of S25 and S35(p<0.05).Each experiment group displayed certain changes except that the S15 group showed the trend of incessantly descending.Meanwhile,the SOD activity of muscle in mud crab increased significantly when transferred to 5,15 and 35(p<0.05).The effect of the muscle SOD activity of Scylla serrata in the experimental group decreased with the time over 72h stress.Whereas the SOD activity in hepatopancreas of mud crabs that transferred from salinity 25 to salinity 5,15 and 35 increased.The influence of salinity stress on the SOD activity of mud crab was significant(p<0.05),relative to the elapsed time.In conclusion,the effects of salinity stress on the non-specific immunity of mud crab would affect activities of PO in serum and SOD in muscle and the hepatopancreas.更多还原