【摘要】 采用间接竞争性酶联免疫吸附法(ELISA)测定滇重楼内生真菌中薯蓣皂苷元的含量。该方法快速、灵敏、操作简便,可以同时检测多个样品,其检测结果与薄层层析方法的检测结果一致,这为高效筛选产薯蓣皂苷元类化合物的内生真菌创造了条件。更多还原

【Abstract】 The content of diosgenin of the endophytic fungi from Paris polyphylla var.yunnanensis was evaluated by an indirect competitive enzyme-linked immunosorbent assay(ELISA) which was proved to be fast,sensitive,easily manipulated and practical to screen multiple samples at one time.The result of the diosgenin content analyzed by ELISA was similar to that by the method of thin layer chromatography.It is very helpful for us to efficiently screen the endophytic fungi with a high content of diosgenin.更多还原